SPELL - S. cerevisiae

Citation	Romero AM, Ramos-Alonso L, Montella-Manuel S, Garcia-Martinez J, de la Torre-Ruiz MA, Perez-Ortin JE, Martinez-Pastor MT, Puig S. A genome-wide transcriptional study reveals that iron deficiency inhibits the yeast TORC1 pathway. Biochimica et biophysica acta. Gene regulatory mechanisms, 2019.
PubMed ID	31394264
Short Description	A genome-wide transcriptional study reveals that iron deficiency inhibits the yeast TORC1 pathway.
# of Conditions	11
Full Description	Iron is an essential micronutrient that participates as a cofactor in a broad range of metabolic processes including mitochondrial respiration, DNA replication, protein translation and lipid biosynthesis. Adaptation to iron deficiency requires the global reorganization of cellular metabolism directed to optimize iron utilization. The budding yeast Saccharomyces cerevisiae has been widely used to characterize the responses of eukaryotic microorganisms to iron depletion. In this report, we used a genomic approach to investigate the contribution of transcription rates to the modulation of mRNA levels during adaptation of yeast cells to iron starvation. We reveal that a decrease in the activity of all RNA polymerases contributes to the down-regulation of many mRNAs, tRNAs and rRNAs. Opposite to the general expression pattern, many genes including components of the iron deficiency response, the mitochondrial retrograde pathway and the general stress response display a remarkable increase in both transcription rates and mRNA levels upon iron limitation, whereas genes encoding ribosomal proteins or implicated in ribosome biogenesis exhibit a pronounced fall. This expression profile is consistent with an activation of the environmental stress response. The phosphorylation stage of multiple regulatory factors strongly suggests that the conserved nutrient signaling pathway TORC1 is inhibited during the progress of iron deficiency. These results suggest an intricate crosstalk between iron metabolism and the TORC1 pathway that should be considered in many disorders.
Tags	metal or metalloid ion stress