SPELL - S. cerevisiae

Citation	Santos-Pereira JM, Garcia-Rubio ML, Gonzalez-Aguilera C, Luna R, Aguilera A. A genome-wide function of THSC/TREX-2 at active genes prevents transcription-replication collisions. Nucleic acids research, 2014.
PubMed ID	25294824
Short Description	A genome-wide function of THSC/TREX-2 at active genes prevents transcription-replication collisions.
# of Conditions	15
Full Description	The THSC/TREX-2 complex of Saccharomyces cerevisiae mediates the anchoring of transcribed genes to the nuclear pore, linking transcription elongation with mRNA export and genome stability, as shown for specific reporters. However, it is still unknown whether the function of TREX-2 is global and the reason for its relevant role in genome integrity. Here, by studying two TREX-2 representative subunits, Thp1 and Sac3, we show that TREX-2 has a genome-wide role in gene expression. Both proteins show similar distributions along the genome, with a gradient disposition at active genes that increases towards the 3' end. Thp1 and Sac3 have a relevant impact on the expression of long, G+C-rich and highly transcribed genes. Interestingly, replication impairment detected by the genome-wide accumulation of the replicative Rrm3 helicase is increased preferentially at highly expressed genes in the thp1Delta and sac3Delta mutants analyzed. Therefore, our work provides evidence of a function of TREX-2 at the genome-wide level and suggests a role for TREX-2 in preventing transcription-replication conflicts, as a source of genome instability derived from a defective messenger ribonucleoprotein particle (mRNP) biogenesis.
Tags	mRNA processing, transcription