SPELL - S. cerevisiae

Citation	de Kok S, Yilmaz D, Suir E, Pronk JT, Daran JM, van Maris AJ. Increasing free-energy (ATP) conservation in maltose-grown Saccharomyces cerevisiae by expression of a heterologous maltose phosphorylase. Metabolic engineering, 2011.
PubMed ID	21684346
Short Description	Increasing free-energy (ATP) conservation in maltose-grown Saccharomyces cerevisiae by expression of a heterologous maltose phosphorylase.
# of Conditions	13
Full Description	Increasing free-energy conservation from the conversion of substrate into product is crucial for further development of many biotechnological processes. In theory, replacing the hydrolysis of disaccharides by a phosphorolytic cleavage reaction provides an opportunity to increase the ATP yield on the disaccharide. To test this concept, we first deleted the native maltose metabolism genes in Saccharomyces cerevisiae. The knockout strain showed no maltose-transport activity and a very low residual maltase activity (0.03 mumol mg protein(-1)min(-1)). Expression of a maltose phosphorylase gene from Lactobacillus sanfranciscensis and the MAL11 maltose-transporter gene resulted in relatively slow growth (mu(aerobic) 0.09 +/- 0.03 h(-1)). Co-expression of Lactococcus lactis beta-phosphoglucomutase accelerated maltose utilization via this route (mu(aerobic) 0.21 +/- 0.01 h(-1), mu(anaerobic) 0.10 +/- 0.00 h(-1)). Replacing maltose hydrolysis with phosphorolysis increased the anaerobic biomass yield on maltose in anaerobic maltose-limited chemostat cultures by 26%, thus demonstrating the potential of phosphorolysis to improve the free-energy conservation of disaccharide metabolism in industrial microorganisms.
Tags	carbon utilization, fermentation