SPELL - S. cerevisiae

Citation	Thierry A, Khanna V, Creno S, Lafontaine I, Ma L, Bouchier C, Dujon B. Macrotene chromosomes provide insights to a new mechanism of high-order gene amplification in eukaryotes. Nature communications, 2015.
PubMed ID	25635677
Short Description	Macrotene chromosomes provide insights to a new mechanism of high-order gene amplification in eukaryotes.
# of Conditions	4
Full Description	Copy number variation of chromosomal segments is now recognized as a major source of genetic polymorphism within natural populations of eukaryotes, as well as a possible cause of genetic diseases in humans, including cancer, but its molecular bases remain incompletely understood. In the baker's yeast Saccharomyces cerevisiae, a variety of low-order amplifications (segmental duplications) were observed after adaptation to limiting environmental conditions or recovery from gene dosage imbalance, and interpreted in terms of replication-based mechanisms associated or not with homologous recombination. Here we show the emergence of novel high-order amplification structures, with corresponding overexpression of embedded genes, during evolution under favourable growth conditions of severely unfit yeast cells bearing genetically disabled genomes. Such events form massively extended chromosomes, which we propose to call macrotene, whose characteristics suggest the products of intrachromosomal rolling-circle type of replication structures, probably initiated by increased accidental template switches under important cellular stress conditions.
Tags	genome variation