SPELL - S. cerevisiae

Citation	Lu S, Lee KK, Harris B, Xiong B, Bose T, Saraf A, Hattem G, Florens L, Seidel C, Gerton JL. The cohesin acetyltransferase Eco1 coordinates rDNA replication and transcription. EMBO reports, 2014.
PubMed ID	24631914
Short Description	The cohesin acetyltransferase Eco1 coordinates rDNA replication and transcription.
# of Conditions	27
Full Description	Eco1 is the acetyltransferase that establishes sister-chromatid cohesion during DNA replication. A budding yeast strain with an eco1 mutation that genocopies Roberts syndrome has reduced ribosomal DNA (rDNA) transcription and a transcriptional signature of starvation. We show that deleting FOB1--a gene that encodes a replication fork-blocking protein specific for the rDNA region--rescues rRNA production and partially rescues transcription genome-wide. Further studies show that deletion of FOB1 corrects the genome-wide replication defects, nucleolar structure, and rDNA segregation that occur in the eco1 mutant. Our study highlights that the presence of cohesin at the rDNA locus has a central role in controlling global DNA replication and gene expression.
Tags	mitotic cell cycle