SPELL - S. cerevisiae

Citation	Kunkel J, Luo X, Capaldi AP. Integrated TORC1 and PKA signaling control the temporal activation of glucose-induced gene expression in yeast. Nature communications, 2019.
PubMed ID	31395866
Short Description	Integrated TORC1 and PKA signaling control the temporal activation of glucose-induced gene expression in yeast.
# of Conditions	21
Full Description	The growth rate of a yeast cell is controlled by the target of rapamycin kinase complex I (TORC1) and cAMP-dependent protein kinase (PKA) pathways. To determine how TORC1 and PKA cooperate to regulate cell growth, we performed temporal analysis of gene expression in yeast switched from a non-fermentable substrate, to glucose, in the presence and absence of TORC1 and PKA inhibitors. Quantitative analysis of these data reveals that PKA drives the expression of key cell growth genes during transitions into, and out of, the rapid growth state in glucose, while TORC1 is important for the steady-state expression of the same genes. This circuit design may enable yeast to set an exact growth rate based on the abundance of internal metabolites such as amino acids, via TORC1, but also adapt rapidly to changes in external nutrients, such as glucose, via PKA.
Tags	carbon utilization, stress