SPELL - S. cerevisiae

Citation	Gibson BR, Lawrence SJ, Boulton CA, Box WG, Graham NS, Linforth RS, Smart KA. The oxidative stress response of a lager brewing yeast strain during industrial propagation and fermentation. FEMS yeast research, 2008.
PubMed ID	18373683
Short Description	The oxidative stress response of a lager brewing yeast strain during industrial propagation and fermentation.
# of Conditions	17
Full Description	Commercial brewing yeast strains are exposed to a number of potential stresses including oxidative stress. The aim of this investigation was to measure the physiological and transcriptional changes of yeast cells during full-scale industrial brewing processes with a view to determining the environmental factors influencing the cell's oxidative stress response. Cellular antioxidant levels and genome-wide transcriptional changes were monitored throughout an industrial propagation and fermentation. The greatest increase in cellular antioxidants and transcription of antioxidant-encoding genes occurred as the rapidly fermentable sugars glucose and fructose were depleted from the growth medium (wort) and the cell population entered the stationary phase. The data suggest that, contrary to expectation, the oxidative stress response is not influenced by changes in the dissolved oxygen concentration of wort but is initiated as part of a general stress response to growth-limiting conditions, even in the absence of oxygen. A mechanism is proposed to explain the changes in antioxidant response observed in yeast during anaerobic fermentation. The available data suggest that the yeast cell does not experience oxidative stress during industrial brewery handling. This information may be taken into consideration when setting parameters for industrial brewery fermentation.
Tags	fermentation, oxidative stress