SPELL - S. cerevisiae

Citation	Shapira M, Segal E, Botstein D. Disruption of yeast forkhead-associated cell cycle transcription by oxidative stress. Molecular biology of the cell, 2004.
PubMed ID	15371544
Short Description	Oxidative stress responses
# of Conditions	70
Full Description	Oxidative stress caused by Menadione or Hydrogen peroxide in synchronized Saccharomyces cerevisiae cultures. Alpha factor synchronized cultures (0.2-0.4 OD), treated at the beginning of S phase (25 min after release from G1 arrest) with either 2 mM Menadione (MD) or 0.24 mM Hydrogen peroxide (HP), show cell cycle effects. Cells treated with MD arrested at G1. Cells treated with HP delayed at S and then, after removal of HP at 135 minutes , continued the cell cycle, only to arrest at G2/M. Growth was carried out in 30C with shaking (295 rpm).
Tags	chemical stimulus, mitotic cell cycle, oxidative stress, stress