SPELL - S. cerevisiae

Citation	Molina-Navarro MM, Castells-Roca L, Belli G, Garcia-Martinez J, Marin-Navarro J, Moreno J, Perez-Ortin JE, Herrero E. Comprehensive transcriptional analysis of the oxidative response in yeast. The Journal of biological chemistry, 2008.
PubMed ID	18424442
Short Description	Oxidative stress response in t-butyl hydroperoxide treated cultures; transcription rate analysis by genomic run-on methodology, 3 replicates
# of Conditions	18
Full Description	The oxidative stress response in Saccharomyces cerevisiae has been analyzed by parallel determination of mRNA levels and transcription rates for the entire genome. A mathematical algorithm has been adapted for a dynamic situation such as the response to stress, to calculate theoretical mRNA decay rates from the experimental data. Yeast genes have been grouped into 25 clusters according to mRNA level and transcription rate kinetics, and average mRNA decay rates have been calculated for each cluster. In most of the genes, changes in one or both experimentally determined parameters occur during the stress response. 24% of the genes are transcriptionally induced without an increase in mRNA levels. The lack of parallelism between the evolution of the mRNA amount and transcription rate predicts changes in mRNA stability during stress. Genes for ribosomal proteins and rRNA processing enzymes are abundant among those whose mRNAs are predicted to destabilize. The number of genes whose mRNAs are predicted to stabilize is lower, although some protein folding or proteasomal genes are among the latter. We have confirmed the mathematical predictions for several genes pertaining to different clusters by experimentally determining mRNA decay rates using the regulatable tetO promoter in transcriptional expression conditions not affected by the oxidative stress. This study indicates that the oxidative stress response in yeast cells is not only conditioned by gene transcription but also by the mRNA decay dynamics and that this complex response may be particularly relevant to explain the temporary down-regulation of protein synthesis occurring during stress.
Tags	oxidative stress, stress