SPELL - S. cerevisiae

Citation	Kitagawa E, Akama K, Iwahashi H. Effects of iodine on global gene expression in Saccharomyces cerevisiae. Bioscience, biotechnology, and biochemistry, 2005.
PubMed ID	16377885
Short Description	Effects of iodine on global gene expression in Saccharomyces cerevisiae.
# of Conditions	27
Full Description	It is well documented that iodine kills microorganisms with a broad spectrum, but a systematic study of its mechanism of action has not yet been reported. Here we found the action of iodine on gene expression level, using the yeast Saccharomyces cerevisiae with a DNA microarray. It was found that, like antimicrobial activity, iodine causes an immediate and dose-dependent (0.5 mM, 0.75 mM and 1 mM) transcriptional alteration in yeast cells. The effects of iodine continued after the first immediate response. Genes for c-compound and carbohydrate metabolism, for energy, and for cell rescue were continuously up-regulated. On the other hand, genes related to protein fate were induced especially at 0.5 h. The gene expression profile at 0.5 h was significantly different from that of a longer iodine exposed condition. The main reaction at 0.5 h after iodine addition might be due to oxidative toxicity, and the profile at 0.5 h was similar to that of an agricultural bactericide.
Tags	chemical stimulus